Title: Preclinical Models Imaging Workshop
Date: 18-22/23 October 2021
Duration: 4-5 days
Registrations: extended until October 14 (cancellation fee: 50€)
Fees:
- ➢ Theory only (online videoconference, max 50 places): 150€
- ➢ Theory & practice (max 30 places, theory online – practice on-site): 250€
COMULIS Scholarships available! *
Theory fees include virtual attendance, access to course materials and dinner night for participants located in Attica at the time. Practice fees include attendance of the practical session, access to additional course materials and light lunch.
At the end of the registration period participants will be notified if they have secured a place for the practical training session and if they are selected for the scholarship, and billing instructions will be forwarded.
*Scholarships: COMULIS announces 10 scholarships, 5 for theory only and 5 for theory & practice attendance option. Scholarships will cover attendance fee or attendance fee and travel expenses respectively. To apply please submit a 200-300-word motivation letter stating your reasons to participate and the expected benefits of your related scientific work during the application process.
Contact: For enquiries and scholarship applications contact azacharioudaki@bioemtech.com
Local Organizers (BIOEMTECH Laboratories): Dr. Argyro Zacharioudaki (AZ), Dr. Maritina Rouchota (MR), Irinaios Pilatis (RP), Prof. George Loudos (GL)
Description:
This workshop aims to present theoretical and practical aspects of preclinical models’ imaging. The workshop provides a general overview of licensing and operating an imaging facility for rodent models, as well as specific details for selecting and applying imaging modalities for various models. The (optional) practical part offers training in mouse handling for imaging projects and operating imaging equipment.
Preliminary Program (hours refer to CET!)
Day 1 (Monday 18/10/2021): Organizing Imaging Projects
09.00 CET Welcome & Ιntroduction (G Loudos & COMULIS Coordinator)
09.10 CET Preclinical imaging facility: goal & operation (20’ – M Rouchota)
09.30 CET Preclinical project management (30’ – C Berthet)
10.00 CET Imaging as a refinement tool (30’ – G Loudos)
Coffee break
Imaging technologies and applications (90’)
(A presentation of core imaging modalities with basic information of the technology each utilizes, the structures each visualizes, particularities and examples of research applications)
11.00 CET MRI (20’ – M Srinivas)
11.20 CET PET-CT (20’ – A Gaitanis)
11.40 CET SPECT-CT (20’- P Chaudhari)
Lunch break
13.20 CET Fluorescence microscopy-based imaging techniques to study cellular and molecular events in preclinical animal models (20’ – M Francolini)
Animal Procedures (90’)
13.40 CET Animal use projects: authorization, ethics and mouse biomethodology (70’ – A Zacharioudaki & TBA)
14.50 CET Intravenous administration of compounds during dynamic imaging (20’ – E Balafas)
Day 2 (Tuesday 19/10/2021): Specific models Part 1
Rodent models for cardiac diseases (90’)
09.00 CET Disease & models (20’ – I Mourouzis)
09.20 CET Imaging applications (20’ – I Mourouzis)
09.40 CET Animal procedures and humane endpoints (20’ – M Katsimpoulas)
10.00 CET Ex vivo studies & sample processing (20’ – M Mavroidis)
Rodent models for neurological diseases (90’)
10.30 CET Intravital imaging of brain inflammation with two-photon microscopy (20’ – D Thomaidou)
10.50 CET Imaging applications in neurodegenerative diseases (20’ – V Kyrargyri)
11.10 CET Animal procedures and humane endpoints (20’ – E Fragkiadaki)
11.30 CET Ex vivo studies & sample processing (20’ – A Tsingotjidou)
Lunch break
Rodent models for liver diseases (90’)
13.00 CET Disease & models (20’ – A Chatzigeorgiou)
13.20 CET Animal procedures and humane endpoints (10’ – ΤΒΑ)
13.30 CET Imaging applications (10’ – A Chatzigeorgiou)
13.40 CET Ex vivo studies & sample processing (20’ – I Pateras)
Plenary lecture
14.30 CET Animal welfare considerations and impact on imaging studies (30’ – J Lopez-Tremoleda)
Day 3 (Wednesday 20/10/2021): Specific models Part 2
Rodent models for oncology (90’)
09.00 CET Disease & models (20’ – I GLADWYN-NG)
09.20 CET Animal procedures and humane endpoints (20’ – L Whitfield)
09.40 CET Imaging applications (20’ – V Ntafis)
10.00 CET Ex vivo studies & sample processing (20’ – M Barbeck)
Rodent models of arthritis (90’)
10.30 CET Disease & models (30’ – M Armaka)
11.00 CET Imaging applications (20’ – M Dragolia)
11.20 CET Experimental procedures and readouts of a spondyloarthritis model (30’ – E Christodoulou)
Lunch break
Rodent models for osteoporosis (90’)
13.00 CET Disease & models (20’ – I Dontas)
13.20 CET Animal procedures, humane endpoints, imaging applications (30’ – P Lelovas)
13.50 CET Imaging applications, ex vivo studies & sample processing (30’ – E Douni)
Day 4 (Thursday 21/10/2021): Practices, Data and Applications
09.00 CET Radiolabelling compounds for imaging and therapy (30’ – P Bouziotis)
09.30 CET Image processing (45’ )
10.45 CET Good Laboratory Practice (30’ – T Karampelas)
Coffee break
11.30 CET Regulatory drug development (30’ – P Glerup)
12.00 CET The next step: EMA/FDA applications (30’ – I Allen)
Lunch break
13.30 CET Imaging in practice (preparation for the workshop) (30’ – I Pilatis)
14.00 CET MCQ Test, Course evaluation and closure of theoretical part
Dinner night for all participants and speakers who reside in Attica
Day 5 (22 or 23/10/2021): Practical training,
Each participant attends one 3h session either on 22/10 or on 23/10.
Sessions: 09.00 – 12.00, 13.00 -16.00 (hours refer to ΕΕSΤ-Athens!)
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- Mouse Workshop (90’ – AZ)
- Mouse handling – practice on live animals.
- Substance administration (iv, sc, ip, og) – practice on “mimicky” mouse.
- Imaging practice (90’ – RP)
- Simulation of use of anesthetic machine and anesthesia during imaging.
- CT, SPECT, β-eye, γ-eye imaging using the mouse phantom model.
- Post-processing examples.
- Mouse Workshop (90’ – AZ)
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Invited Speakers & Laboratories
Irving Allen, MBA, MS, PhD, President and Director of Research Consulting, Allen Consulting and Management
Marietta Armaka, PhD, Researcher C’ (equivalent to assistant professor level), BSRC “Alexander Fleming”
Evangelos Balafas, BSc, MSc, Senior LATG, Center of Clinical, Experimental Surgery and Translational Research, Biomedical Research Foundation of the Academy of Athens
Mike Barbeck, PhD, Managing Director, BerlinAnalytix
Cyril Berthet, PhD, Pharmaco-imaging Unit Director, Oncodesign
Penelope Bouziotis, PhD, Research Director, Radiochemical Studies Laboratory, Institute of Nuclear & Radiological Sciences & Technology, Energy & Safety INRASTES, NCSR “Demokritos”
Antonios Chatzigeorgiou, MD, PhD, Assistant Professor of Experimental Physiology, Department of Physiology, Medical School, National and Kapodistrian University of Athens
Pradip Chaudhari
Eleni Christodoulou, PhD, Biomedcode Hellas
Ismene Dontas, DVM, PhD, Professor of Experimental Surgical Research, Laboratory for Research of the Musculoskeletal System, School of Medicine, National and Kapodistrian University of Athens
Eleni Douni, Associate Professor, Department of Biotechnology, Agricultural University of Athens & Associate Researcher, Biomedical Sciences Research Center “Alexander Fleming”
Melina Dragolia, MSc, Technician, Animal Facilities BSRC “Alexander Fleming”
Eirini Fragkiadaki, DVM, MSc, PhD, Head of Laboratory Animals Facilities, Designated veterinarian, Department of Animal Models for Biomedical Research, Hellenic Pasteur Institute
Maura Francolini, PhD, Dept. of Medical Biotechnology and Translational Medicine, Universita degli Studi di Milano
Ivan E. GLADWYN-NG, B.Sc.(Med.), Ph.D. , Field Application Scientist, Regional Lead EMEA & APAC, Taconic Biosciences
Peter Glerup, DVM, MSc, Senior Director of Non-Clinical Safety Assessment, Zealand Pharma A/S
Theodoros Karampelas, PhD, Quality Assurance Manager, BIOEMTECH Laboratories
Michalis Katsimpoulas, DVM, SRS, PhD, Staff Research Scientist – Assistant Professor Level, Experimental Surgical Unit, Center of Clinical, Experimental Surgery and Translational Research, Biomedical Research Foundation of the Academy of Athens
Vasiliki Kyrargyri, PhD, Independent Postdoctoral Researcher, ELIDEK fellow, Department of immunology, Laboratory of Molecular Genetics, Hellenic Pasteur Institute
Pavlos Lelovas, DVM, MSc, PhD, Designated Veterinarian, Laboratory for Research of the Musculoskeletal System, School of Medicine, National and Kapodistrian University of Athens
Jordi L.Tremoleda, DVM, PhD, FHEA, MRCVS, Named Veterinary Surgeon and Welfare Officer, Senior Lecturer in Trauma, Animal Science & Welfare, Blizard Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University of London
George Loudos, PhD, Assistant Professor, Department of Biomedical Engineering, University of West Attica, Athens, Greece & CEO, BIOEMTECH Laboratories
Manolis Mavroidis, PhD, Investigator – Assistant Professor Level, Center of Basic Research, Biomedical Research Foundation Academy of Athens
Iordanis Mourouzis, Associate Professor, Dpt of Pharmacology, Medical School, National and Kapodistrian University of Athens
Vasileios Ntafis, DVM, MSc, PhD, Staff Scientist B’, Head-Designated Veterinarian, Animal Facilities BSRC “Alexander Fleming”
Ioannis Pateras, PhD
Irenaios Pilatis, Laboratory Engineer, BIOEMTECH Laboratories
Maritina Rouchota, PhD, Lab Manager & Application Specialist, BIOEMTECH Laboratories
Mangala Srinivas, PhD, FYAE, Professor and Head of Dept. – Chair Group Leader, Cell Biology & Immunology (CBI), Wageningen University & Research
Dimitra Thomaidou, PhD, Research Director, Laboratory of Cellular and Molecular Neurobiology, Hellenic Pasteur Institute
Anastasia Tsingotjidou, Associate Professor, Laboratory of Anatomy, Histology and Embryology, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki
Lucy Whitfield, MA VetMB DLAS PGCert(VetEd) FHEA MRCVS, Director, OWL Vets Ltd
Argyro Zacharioudaki, DVM MLAS DiplECLAM, Veterinarian & In-vivo Research Consultant, BIOEMTECH Laboratories
